domain maps of the pde11a protein  (InterPro Inc)

Journal: PLoS ONE

Article Title: miR-181 interacts with signaling adaptor molecule DENN/MADD and enhances TNF-induced cell death

doi: 10.1371/journal.pone.0174368

Figure Lengend Snippet: A) Luciferase reporter construct containing the 3'-UTR of human MADD mRNA. B) The miR-181a and miR-181b sequence alignments with their predicted target site in 3’UTR of MADD mRNA are presented. C). Homology between miRNA binding site at 3’UTR of MADD between human and mouse D) miR-181a and miR-181b mature miRNA sequences homology in human and mouse.

Article Snippet: Equal amounts of protein (50Mg) from different cells were used to measure DENN/MADD level with Mouse MAP kinase-activating death domain protein, MADD ELISA Kit (Mybiosource).

Techniques: Luciferase, Construct, Sequencing, Binding Assay

Journal: PLoS ONE

Article Title: miR-181 interacts with signaling adaptor molecule DENN/MADD and enhances TNF-induced cell death

doi: 10.1371/journal.pone.0174368

Figure Lengend Snippet: Primer sets used in this study.

Article Snippet: Equal amounts of protein (50Mg) from different cells were used to measure DENN/MADD level with Mouse MAP kinase-activating death domain protein, MADD ELISA Kit (Mybiosource).

Techniques:

Journal: PLoS ONE

Article Title: miR-181 interacts with signaling adaptor molecule DENN/MADD and enhances TNF-induced cell death

doi: 10.1371/journal.pone.0174368

Figure Lengend Snippet: Luciferase assay confirms the interaction of miR-181b with DENN/MADD transcript. Co-transfection of HEK293T cells with vectors encoding the 3'UTR of DENN/MADD ligated to the Renilla luciferase with microRNA mimic sequences and negative control sequence showed significant decrease of luciferase activity in cells transfected with miR-181 b mimic sequences. Renilla luciferase levels were normalized against internal Firefly luciferase. Data are shown as means ± SEM ( n = 5). ANOVA, Tukey post hoc (**p≤0.01 relative to negative control)

Article Snippet: Equal amounts of protein (50Mg) from different cells were used to measure DENN/MADD level with Mouse MAP kinase-activating death domain protein, MADD ELISA Kit (Mybiosource).

Techniques: Luciferase, Cotransfection, Negative Control, Sequencing, Activity Assay, Transfection

Journal: PLoS ONE

Article Title: miR-181 interacts with signaling adaptor molecule DENN/MADD and enhances TNF-induced cell death

doi: 10.1371/journal.pone.0174368

Figure Lengend Snippet: L929 cells were transfected with miR-181a, miR-181b mimics, inhibitors and negative control (50nM) for 24 or 48 hours. Cells were also treated with TNF-α (50ng/ml) for 30 minutes after transfection. mRNA expression A) protein level B) of DENN/MADD in transfected cells are shown by RT-PCR and ELISA, respectively. Data are shown as means ± SEM. (n = 3) Kruskal wallis & ANOVA, Tukey post hoc (* p<0.05, **p<0.01, relative to negative control).

Article Snippet: Equal amounts of protein (50Mg) from different cells were used to measure DENN/MADD level with Mouse MAP kinase-activating death domain protein, MADD ELISA Kit (Mybiosource).

Techniques: Transfection, Negative Control, Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay